Ziehl-Neelsen stain


'''Ziehl-Neelsen stain''' is used to stain acid-fast mycobacteria. It can also be used to stain few other bacteria like Nocardia. The reagents used are Ziehl-Neelson carbol fucshin, acid alcohol and methylene blue.

Procedure

# Add Ziehl-Neelson carbol fucshin to the slide for five minutes while applying heat. # This is follwed by a gentle wash with water to cool the slide. # Acid alcohol is now added to decolorize the slide. # Wash the slide in water again and counterstain with methylene blue for one to two minutes. # The acid-fast bacteria retain the red color, and are therefore seen red against a blue background. Category:Microbiology
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